Merck, 90020508-DNA-5UG, 1BR.3.N

Application
DNA repair and cell growth studies
Biochem/physiol Actions
Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmid pSV2neo. The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.
Human skin fibroblast, transformed
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 9,12
D16S539: 12,14
D5S818: 12
D7S820: 8,10
THO1: 9.3
TPOX: 11
vWA: 14,15
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].