Merck, 90071806-DNA-5UG, ARLJ 301-3

Application
Transformation studies
Biochem/physiol Actions
A spontaneous transformant of the cell line ARLJ 301-3 from the F334/Ducrj rat.
Rat F334/Ducrj liver
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
William′s E + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].