Merck, 90071808-DNA-5UG, Anr4

Application
Transformation studies, analysis of metabolic activity
Biochem/physiol Actions
EJ-ras oncogene transformed ARLJ301-3 cell line (Sigma Catalogue number. 90071806) from a 10 week old F334/Ducrj rat liver.
Rat F344/Ducrj liver
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
William′s E + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].