Merck, 90092701-DNA-5UG, 3T3-MSV

General description
Product has lost contact inhibition
Application
Cell growth studies
Biochem/physiol Actions
Mouse Swiss 3T3 embryo, Moloney-MSV transformed
This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.
Preparation Note
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].