Merck, 91060554-1VL, 73B

Immunogen
Sheep lymphocytes
Application
Useful for distinguishing naive and memory T cells, FACS
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
The antibody is directed against the p220 component of the sheep CD45 molecule (CD45RA), expressed on all B cells and a sub-population of naive T cells.
Preparation Note
EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].