Merck, 91060557-1VL, 86D

Immunogen
Sheep CD4- CD8- T lymphocytes
Application
Enumeration of the gamma/delta T cell subset in ruminants
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
The antibody is directed against the sheep gamma/delta T cell receptor (TcR) expressed on approximately 20% of PBL and 3% of thymocytes. There is species cross-reactivity with goat, cattle and pig (low affinity).
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].