Merck, 92101203-DNA-5UG, B16-F1 Cell Line from mouse

Application
B16-F1 cell line from mouse has been used to study the cytotoxic and antioxidant properties of supercritical CO2 extract (SCE) of old man′s beard (Usnea barbata). It has also been used to study the antiproliferative activity of essential oils from Achillea ligustica.
Biochem/physiol Actions
Mouse melanoma which produces melanin.
Mouse melanoma, producing melanin
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].