Merck, 93040110-DNA-5UG, A15

Application
Tumourigenicity studies
Biochem/physiol Actions
A renamed subculture of the rat gliomal line 1A2(R) - 93040129. A15 is tumourigenic in BDIX rats.
Rat, BDIX, glioma
Preparation Note
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm² flask using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C. Cells can reach up to 4x1,000,000 cells per 25cm² flask.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].