Merck, 93040121-DNA-5UG, BE11 (Early)

Application
Study of carcinogenesis
Biochem/physiol Actions
Rat, BDIX, foetal brain
The BE11 culture was derived 2 days after a single injection of buffer into pregnant rats. The cells do not form colonies in agar and are not tumourigenic in BDIX rats. Plasminogen activator activity is barely detectable. They are suitable controls for BE10 (Early) cells (93040118).
Preparation Note
DMEM + 2mM Glutamine + 15-20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x100,000 cells/cm² using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C. Cultures should reach 2x1,000,000 cells per 25cm² flask. Subculture weekly.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].