Merck, 93120840-DNA-5UG, BHK 21 STRAIN 31

Application
Virus studies
Biochem/physiol Actions
Hamster Syrian kidney
Used extensively for the production of foot and mouth disease virus.
Preparation Note
GMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].