Description
General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Application
Triton™ X-100 has been used:for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
As a component of lysis buffer used for homogenization of cells
As a component of blocking buffer in immunofluorescence staining
For the preparation of cells in double fluorescence staining
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Features and Benefits
Suitable for Molecular Biology and Biochemical Research
Free from DNase, RNase, NICKase and protease
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
High purity product for research applications
Tested for the presence of Anionic and Cationic traces
Other Notes
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Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow