Merck, 94042254-DNA-5UG, B16 melanoma 4A5 Cell Line from mouse

Application
B16 melanoma 4A5 cell line has been used to study the spectral and biological characteristics of copper (II) mesoporphyrinic complexes. It has also been used to evaluate the cytotoxicity of JaZ-30, a matrix metalloproteinase-2 (MMP-2) inhibitor.
Biochem/physiol Actions
Derived from a melanoma from the skin of a C57BL/6 strain mouse, showing fibroblast-like characteristics, which produces melanin. Cells may lose ability to produce pigmentation in long term culture.
Mouse melanoma, melanin producing
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].