Merck, 94050507-1VL, B3/25

Immunogen
K-562 cells
Biochem/physiol Actions
Mouse S194/5.XXO.BU.1 x Mouse spleen cells
The antibody is directed against an acidic glycoprotein (dimer of 2x110kDa monomers joined by disulphide bonds) present on immature and proliferating human haematopoietic cells but not circulating peripheral blood cells, or other non haematopoietic cells.
Preparation Note
DMEM + 2mM glutamine + 0.02mM 2-mercaptoethanol (2ME) + 10% Horse Serum.
Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].