General descriptionConsanguineous: No
Homozygous: No
Biochem/physiol ActionsCw: 501
HLA DRB1: 0404 1401
HLA DRB3: 202
HLA DRB4: 101
HLA DQAP: 1.3 3.1
HLA DQA1: 0101 03
HLA DQB1: 0302 0503
HLA DPB1: 01011 0301
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation NoteRPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
HLA A: 2 31
HLA C: 3 5
HLA B: 44 60
Bw4/bw6: 4 6
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Only the genomic DNA extracted from the cell line is available for purchase by commercial organizations, and not the cell line itself. No licensing agreement is required when the DNA extracted from this cell line are used for internal research purposes only. Frozen cell cultures can be supplied only to academic and not-for-profit organisations. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding other authorized use of this cell line or its genomic DNA may be directed to
[email protected].
DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
