Merck, 94101449-DNA-5UG, AT-1

Biochem/physiol Actions
Derived from a spontaneous Dunning R-3327 rat prostatic cancer. The cells have been shown to be anaplastic with low metastatic ability and non-responsive to androgen.
Rat Dunning R-3327,dorsal prostatic adenocarcinoma
Preparation Note
RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4 x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].