Merck, 94101452-DNA-5UG, AT6.1

Application
Used in studies in which micro-cell mediated chromosomal transfer of human chromosome 11 suppressed metastatic ability. This suppresser function was localised to 11p11.2-13.
Biochem/physiol Actions
A highly metastatic, anaplastic, androgen-independent sub-line which spontaneously developed during the serial passage of the non-metastatic, well-differentiated, androgen-responsive R-3327-H sub-lines in the laboratory of Dr J Schalken, Nijmegan, The Netherlands.
Rat dorsal prostatic adenocarcinoma
Preparation Note
RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].