ApplicationUsed in HCV typing studies.
Biochem/physiol ActionsDerived from a patient with chronic Hepatitis C (HCV type 1). Antibody specific to non structural protein 4a (NS4a) of Hepatitis C virus. Fine specificity mapped to amino acid residues 1697-1708 of HCV deduced amino acid sequence. The cells should be handled under laboratory containment level 2 conditions.
Human anti-hepatitis C virus IgG1 secreting B cell
Preparation NoteMaintain cultures between 3-9x10,000 cells/ml; using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
RPMI 1640 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 20u/ml IL-6 + 10% Foetal Bovine Serum (FBS).
Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by any organization may require a license agreement. Customers will be contacted by
[email protected] to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by
[email protected] to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to
[email protected].
DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
