Merck, 94120829-1VL, AFRC 84B4

Immunogen
Influenza A equine 2 virus
Application
Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.
Biochem/physiol Actions
Horse sub-mandibular lymph node x AFRC 17D1
The heterohybridoma AFRC 84B4 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 84B4 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].