Merck, 95030301-1VL, BC9

Immunogen
Bovine recombinant TNF-alpha
Application
Detection of TNF in bioassay or radioimmunoassay, in vivo studies (neutralisation of TNF).
Biochem/physiol Actions
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
The monoclonal antibody BC9 detects bovine tumour necrosis factor (TNF) in a radioimmunoassay with a detection limit of 24 pg/ml. Bovine recombinant TNF can be neutralised in vitro by addition of BC9. Cytotoxic assays showed a neutralising effect using L929 and WEHI 164 clone 13 cells.
Preparation Note
Maintain cultures between 5-6x100,000 cells/ml; 5% CO₂; 37°C.
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].