Merck, 96020930-DNA-5UG, AsPC-1

Biochem/physiol Actions
Human pancreas adenocarcinoma ascites metastasis
This cell line was derived from nude mouse xenografts initiated with cells from the ascites of a 62-year-old female Caucasian patient with cancer of the pancreas. The cells produce carcinoembryonic antigen (CEA), human pancreas associated antigen, human pancreas specific antigen and mucin.
Preparation Note
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].