Merck, 97040308-DNA-5UG, 55-6

Immunogen
rgp 120
Application
gp120 assays
Biochem/physiol Actions
Mouse x Mouse hybridoma
The hybridoma cell line 55-6 produces antibodies reacting with the Human Immunodeficiency Virus I (HIV-1) envelope protein, gp120. Spleen cells from BALB/c mice that had been sequentially immunised with recombinant gp120(rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB,SF2 and Z6) have been fused with Sp2/0-Ag14 myeloma cells. The antibody binds rgp120 from the III B isolate and reacts weakly with gp120 from the MN strain, both shown by ELISA. No cross blocking of any other antibodies in the developed panel was detected, thus indicating that 55-6 binds to a unique site on gp120IIIB. In peptide recognition assays it was shown to bind a synthetic peptide 105-117 (in the CI region) of gp120.
Preparation Note
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO₂;37ºC
RPMI 1640 + 2mM glutamine + HT + 10% FBS
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].