Merck, 99011334-DNA-5UG, AFRC IAH-CC62

Immunogen
Bovine thymocytes
Application
Immunohistology, Flow cytometry, Immunoprecipation
Biochem/physiol Actions
Mouse x Mouse hybridoma
The hybridoma IAH-CC62 secretes antibodies reactive with bovine CD26 - this was formerly known as WC10. This non-lineage specific antigen is present on bovine leukocytes and has a MW of 115 kDa and 39kDa. Cells have been grown on mouse macrophage feeder cells using hypoxanthine and thymidine at concentractions appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
Preparation Note
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO₂; 37°C.
RPMI 1640 + 2 mM glutamine + HT supplement + 10% FBS
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].