Merck, 99011337-DNA-5UG, AFRC IAH-CC98

Immunogen
Bovine thymocytes
Application
Immunohistology, Flow cytometry, Immunoprecipitation
Biochem/physiol Actions
Mouse x Mouse hybridoma
The monoclonal antibody IAH-CC 98 is part of a cluster known as WC6 that immunoprecipitated a molecule of 210 kDa. This molecule is present on all CD2+ cells and B cells, but not expressed by WC1+cells. Cells have been grown on mouse macrophage feeder cells using hypoxanthine and thymidine at concentrations appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C
RPMI 1640 + 2mM glutamine + HT supplement + 10% FBS
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].