Merck, 99072801-DNA-5UG, 10T½

Biochem/physiol Actions
Clone 8, contact inhibited, semi-normal.
Mouse embryo fibroblast
Preparation Note
BME + 10% FBS.
Split sub confluent cultures (70-80%) 1:3 ie. seeding at 3x10,000 cells/cm² using 0.25% trypsin/EDTA. 37°C; 5% CO₂
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].