Description
General description
AnteoTech identified the following problems that researchers are having with their multiplex assays:
Binding difficult antibodies and antigens to microspheres
Complex protocols for inexperienced users
Lengthy microsphere and protein preparation times prior to protein coupling
Reproducibility between batches of protein coupled microspheres
Limited storage of ready-to-couple microspheres
From these findings, they developed the AnteoBind™ Activation Kit For Multiplex Microspheres that is for use with the Luminex xMAP® system and contains the patented AnteoBind Activation Reagent.
With this Activation Kit, you can couple multiple antibodies without extra optimization for each, saving assay development time and reducing the barriers of assay transfer between diverse platforms.
AnteoBind is water based and replaces the dry chemicals you would use with the EDC method. Using AnteoBind instead of NHS/EDC chemistry can save up to 3-4 hours of lab time. Experiments have shown increased stability and functionality for antibodies and a decreased background. AnteoBind improves reproducibility between experiments by providing uniformity between surfaces. You can store your AnteoBind activated microspheres for up to 1 year.
POSTER: A Novel User Friendly Chemistry to Couple Antibodies and Challenging Proteins to Luminex MagPlex Microspheres
Application
Activation and coupling of Luminex Microspheres for Multiplex Immunoassays
Features and Benefits
Water based AnteoBind™ activation and coupling reagent for microspheres
Fast assay development time making it easy to move your ELISA assay to the Luminex xMAP® system
Compatible with both Luminex MagPlex® and MicroPlex® microspheres
Test problematic proteins on xMAP, including those cited as not suitable for the system
An alternative to traditional chemistries such as NHS/EDC methods with an easier to use AnteoBind protocol
Ease of use, simply pipette AnteoBind Reagent to your chosen microspheres to activate
Reduce reagent preparation time by 3-4 hours
Increase antibody functionality
Low background noise
High reproducibility between experiments
Legal Information
Anteobind is a trademark of AnteoTech Ltd
MagPlex is a registered trademark of Luminex Corp
MicroPlex is a registered trademark of Luminex Corp
xMAP is a registered trademark of Luminex Corp
