Description
General description
AnteoBind Sub-Micron was developed for binding antibodies and other proteins magnetic particles from 0.5 μm to 1 μm in size. It forms strong multi-valent interactions with almost any electron donating groups including carboxylic acids. Once proteins are added, AnteoBind activated particles rapidly form very strong but gentle binding interactions in the order of minutes.
AnteoBind Sub-Micron has been used to stably activate many particles. Examples include MyOne Dynabeads®, Estapor® M1 and EM1 particles.
AnteoBind Sub-Micron has many advantages over existing methods such as EDC/NHS chemistry or alternative ″pre-activated″ methods such as Epoxy or Tosyl. AnteoBind is water based and replaces the dry chemicals you would use with the EDC method. Using AnteoBind instead of NHS/EDC chemistry can save up to 3-4 hours of lab time. Experiments have shown increased stability and functionality for antibodies and a decreased background. AnteoBind improves reproducibility between experiments by providing uniformity between surfaces. For particles larger than 1 ·m the recommended AnteoBind product is AnteoBind Micro.
Application
Activation and coupling of particles 0.5 μm to 1 μm in diameter
Features and Benefits
Water based, activation and coupling reagent for particles 0.5 μm to 1 μm in diameter
An alternative to traditional chemistries such as NHS/EDC methods with an easier to use AnteoBind protocol
Reduce reagent preparation time by 3-4 hours
Increase antibody functionality
Low background noise
High reproducibility between experiments
Legal Information
Anteobind is a trademark of AnteoTech Ltd
Dynabeads is a registered trademark of Invitrogen Dynal AS Corp
Estapor is a registered trademark of Merck KGaA, Darmstadt, Germany
