Merck, A1520-1MG, α-Amylase Inhibitor from Triticum aestivum (wheat seed)

General description
α-Amylases are endoglycosidases, that are classified into glycosyl hydrolase family 13 (1-3).
Application
α-Amylase Inhibitor from Triticum aestivum (wheat seed) has been used to determine its effects on salivary gland (SG) amylase activity. It has also been used to inhibit the activity from the extract from Bemisia tabaci.
Biochem/physiol Actions
Competitive inhibitor of human salivary α-amylase. KI = 2.9 nM, compared to a KM of 5.9 mM (calculated per mole of α-1,4-linked maltose residues).
α-Amylases can hydrolyze α-(1,4)-D-glycosidic linkages. It participates in the breakdown of starch and glycogen. Inhibiting this enzyme can be used as a strategy to treat dental caries, diabetes, obesity and periodontal diseases.
Physical form
Lyophilized powder containing buffer salts as sodium phosphate.
Other Notes
One unit will reduce the activity of two units of α-amylase (A0521) by 50% after pre-incubation at 25 °C.