Merck, A1603-100MG, Alginate Lyase

General description
Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.
Application
Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .
Alginate Lyase has been used:
in the pre-processing of enzyme
to de-encapsulate cells
to digest Pseudomonas exopolysaccharide to facilitate electroporation

Biochem/physiol Actions
Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.
Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.
Preparation Note
Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.
Other Notes
One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C
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