Merck, A1805-20EA, ACCUSPIN™Tubes Sterile, 12 mL Capacity

General description
ACCUSPIN™ Tubes are polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 3mL of the density gradient.
Application
ACCUSPIN™ Tubes Sterile, 12 mL Capacity is intended for use with the density gradient separation medium Histopaque™ -1077:

• in the isolation of lymphocytes and other mononuclear cells.
• isolation of cord blood–derived endothelial progenitor cells.citation
• isolate mononuclear cell layer from bone marrow samples.
• suitable for the purification of human bone marrow aspirates for the investigation of the therapeutic value of BET inhibitors for acute myeloid leukemia.
• isolation of cord blood-derived endothelial progenitor cells

Biochem/physiol Actions
Following the addition of Histopaque-1077 to the ACCUSPIN Tube, a brief centrifugation places the Histopaque-1077 below the frit. The blood sample can be added to the top chamber of the tube without the risk of mixing with the Histopaque-1077 in the lower chamber under the frit. On subsequent centrifugation the whole blood descends through the frit to contact with theHistopaque-1077. The elements of greater density displace a volume of Histopaque-1077 above the forgiving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become lightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN Tube. Lymphocytes and other mononuclear cells, e.g., monocytes, remain at the plasma/Histopaque-1077interface. This dense band of mononuclear cells maybe collected by pouring off the contents of the upper chamber or by means of a pipette. Erythrocyte contamination is avoided due to the barrier between the chambers.
Preparation Note
Package contains 20 tubes
Legal Information
Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany