Merck, A4487-1ML, Aphidicolin

General description
Aphidicolin is a tetracyclic diterpene with antiviral and antimitotic properties used for cell cycle synchronization in various cell lines.
Application
Aphidicolin, from Nigrospora sphaerica, was used for cell cycle synchronization of tobacco BY-2 cells. It is used to block the entry of cells into S-phase.
Biochem/physiol Actions
Aphidicolin specifically inhibits DNA polymerase α that is responsible for DNA replication. It also inhibits α-like DNA polymerases of plants and yeasts but does not inhibit synthesis of RNA and proteins. Aphidicolin competes for the dCTP-specific binding site on DNA polymerase α specifically. Aphidicolin has antiviral and antimitotic properties. It blocks the cell cycle at S phase. It has been shown to induce apoptosis in HeLa cells.
Packaging
1 ml
Physical form
Aphidicolin Ready Made Solution supplied as a 1 mg/ml solution in DMSO.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Storage class (TRGS 510): Combustible liquids