Merck, A6861-10UG, Acetyl-CoA carboxylase 2 human

Name: Acetyl-CoA carboxylase 2 human
Brand: Merck
SKU: A6861-10UG

Size:10 μg, ACACB, ACC2, acetyl-CoA carboxylase beta, recombinant, expressed in Sf9 cells

Catalog Number: A6861-10UG

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Category

Recombinant Enzymes

Pack Size10 µg

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
C-terminal HIS-tagged 277 kDa full length protein
Application
Acetyl-CoA carboxylase is responsible for synthesis of Malonyl-CoA which is an inhibitor of fatty acid oxidation in skeletal muscle mitochondria. The enzyme may be used to study the effect on production of malonyl-CoA as well as fatty acid oxidation during exercise. The enzyme also may be used for ACC regulation study in anti-obesity and anti-type 2 diabetes therapeutics.
Biochem/physiol Actions
Acetyl-CoA Carboxylase (ACC) regulates the metabolism of fatty acids. This enzyme catalzes the formation of Malonyl CoA through the irreversible carboxylation of acetyl CoA. There are two main isoforms of Acetyl-CoA carboxylase expressed in mammals, Acetyl-CoA carboxylase 1 (ACACA) and Acetyl-CoA carboxylase 2 (ACACB). ACACA has broad tissue distribution but is enriched in tissues critical for fatty acid sythesis such as adipose tissue. ACACB is enriched in tissues such as skeletal muscle and heart that are critical for fatty acid oxidation.

The Acetyl-CoA Carboxylase enzymes are activated by citrate, glutamate, and dicarboxylic acids and negatively regulated by long and short chain fatty acyl CoAs. Because of thier roles in fatty acid metabolism and oxidation, ACACA and ACACB are therapeutic targets for treating obesity and metabolic syndrome disorders.
Physical form
Buffered aqueous solution containing Tris-HCl, pH 8.0, NaCl, glycerol, and DTT. May also contain one or more of the following: EDTA, KCl, imidazole, TWEEN-20.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit will cause the carboxylation of 1 picomole of acetyl-CoA per minute at pH 7.4 at 30 deg C.

Specifications

General

UNSPSC Code	12352204
NACRES	NA.26
Enzyme Commission (EC) Number	6.4.1.2 ( BRENDA \| IUBMB )
recombinant	expressed in Sf9 cells
form	solution
specific activity	≥25 units/μg protein
NCBI accession no.	NM_001093
shipped in	dry ice
storage temp.	−70°C
Quality Level	200
Gene Information	human ... ACACB(32)

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Merck, A6861-10UG, Acetyl-CoA carboxylase 2 human

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