Merck, A9528-5G, Amphotericin B solubilized

General description
Chemical structure: polyene
Application
Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.
Biochem/physiol Actions
Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.
Packaging
Solubilized Amphotericin B is packaged in amber glass bottle.
Preparation Note
Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.

It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).
Other Notes
After reconstitution with water, the product will be a suspension of Amphotericin B, containing deoxycholate as the solubilizing agent. The lyophilized formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.
Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.
The actual solid weight of the packaged product is calculated according to the UV purity.
Disclaimer
Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.