Description
General description
Histone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone 3.1 is also shown to undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. It has been reported that Histone H3 can also undergo dopaminylation ((H3Q5dop), which can potentiate the binding of adjacent histone post-translational modification interacting proteins and play a vital role in dopaminergic neuronal transcription. This rabbit polyclonal antibody specifically binds to Histone H3 dopaminylated on glutamine 5. (Ref.: Lepack, AE., et al (2020). Science. 368(6487); 197-201).
Immunogen
KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 with dopaminylated glutamine 5.
Application
Anti-H3Q5dopaminyl, Cat. No. ABE2588, is a rabbit polyclonal antibody that detects Histone H3 dopaminylated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.
Western Blotting Analysis: A 1:200 dilution from a representative lot detected H3Q5dopaminyl in human ventral tegmental area (Courtesy of Ian Maze Lab; Icahn School of Medicine at Mount Sinai, New York).
Dot Blot Analysis: A representative lot de
Analysis Note
Evaluated by Western Blotting with recombinant H3 dopaminyated on glutamine 5.
Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant Histone H3 dopaminylated on glutamine 5 with 2.5 mM dopamine, but not with one treated with transglutaminase 2 (0.5 mg).
Other Notes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
