Description
General description
Mouse Interferon regulatory factor 7 or IRF-7 (UniProt P70434) is encoded by the murine irf7 gene (Entrez Gene ID 54123). IFN-γ-induced gene activations are mediated by both signal transducers and activators of transcription (STATs) and interferon regulatory factors (IRFs). STAT1 dimer, for example, stimulates immediate transcription by binding IFN-γ-activated site (GAS) sequence in the promoter region of target genes. While a delayed, secondary transcription activation is observed among IFN-γ target genes containing an IFN regulatory factors (IRF) binding site within the interferon-stimulated response element (ISRE) sequence. Irf7 transcription is shown to be activated by STAT1 and IRF9 in response to type II interferon (IFN) IFN-γ stimulation in MEF cultures. The expressed IRF7 protein then further cooperates with STAT1 and IRF1 to stimulate the expression of a subset of IFN-γ-induced STAT1 target genes. Cat. No. ABF130, Anti-IRF7 is a rabbit polyclonal antiserum raised against recombinant GST fusion protein encompassing murine IRF7 C-terminal half sequence. This antiserum specifically detects INF-gamma-induced IRF7 expression and chromatin association in Wt, but not Irf7-/- MEF by Western blotting and ChIP application, respectively. Murine IRF7 has a calculated M.W. of 51.2 kDa, but migrates with an apparent M.W. of ~60 kDa by SDS-PAGE.
Immunogen
GST-tagged recombinant protein corresponding to the C-terminal of mouse IRF7.
Application
Anti-IRF7 Antibody is an antibody against IRF7 for use in Western Blotting, Chromatin Immunoprecipitation .
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated IRF7-associated DNA fragments using chromatin prepared from infected (12 hours with Vesicular Stomatitis Virus/VSV) or uninfected MEFs cells (Thomas Decker, Max F. Perutz Lab
Analysis Note
Evaluated by Western Blotting in MEF-1 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected IRF7 in 10 ug of MEF-1 cell lysate.
Other Notes
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