Merck, ABN1032, Anti-phospho-Axin-1 (Thr485) Antibody

General description
Linear peptide corresponding to a mouse axin-1 sequence containing phosphorylated Thr485.
~115 kDa observed.
Application
Epitope: Axin-1 pThr485.
Biochem/physiol Actions
This polyclonal antibody detected CDK5/p25-dependent axin-1 Thr485 phosphorylation both in vitro and in vivo (Fang, W.Q., et al. (2011). J. Neurosci. 31(38):13613–13624). The Thr485 designation was originally assigned based on mouse (NP_001153070.1) and rat (NP_077381.1) sequences deposited in NCBI protein database. The numbering can vary depending on species and the source of published sequence. For example, the target resiude corresponds to Thr481 of the human sequence and Thr480 of the mouse and rat sequences reported by UniProt (O15169, O35625 and O70239, respectively).
Physical form
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human fetal brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Thr485-phosphorylated Axin-1 in 10 µg of human fetal brain tissue lysate.
Other Notes
Mouse, Human. Predicted to react with Rat and Bovine based on 100% sequence homology.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.