Description
General description
Product Source: Human plasma, fresh, non-frozen. Tested negative for anti-HIV-1, anti-HIV-2, anti-HCV, anti-HBc and HBsAg.
Two forms of Apo B exist: Apo B-100 and Apo B-48. The first is found in VLDL and LDL and is produced by the liver. The second is found in chylomicrons and originates in the intestine.
Apo B is purified from LDL isolated by density gradient ultracentrifugation (1.03-1.05 g/mL density range) followed by size exclusion chromatography in 10 mM deoxycholate, 50 mM NaCl, 50 mM Na2CO3, pH 10 (Walsh & Atkinson 1983).
PROPERTIES:
Apo B, which has a Mr of 550 kD, is the major protein found in LDL and is responsible for the cellular uptake of LDL particles from the plasma (Goldstein & Brown 1982). The Apo B prepared by this procedure is aggregated. The aggregates are not retained by a 0.45 mm filter, but migrate only a short distance into a 5% SDS PAGE gel. In the isolation buffer, Apo B gives a stable solution that is slightly hazy.
Physical form
Lyophilized from 50 mM Na2CO3, pH 10, 50 mM NaCl, and 10 mM sodium deoxycholate solution. Non-sterile.
Preparation Note
Maintain lyophilized material at <= -20°C for up to six months. Reconstitute to 1 mL by adding deionized water. If the deoxycholate is removed, Apo B-100 will slowly aggregate and become insoluble. After reconstitution maintain at +2-8°C for up to 4 weeks.
Analysis Note
Single arc by IEP against antisera to whole human serum.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
