Merck, AP1145-100UG, Anti-IL13RA2 Mouse mAb (2E10)

General description
Purified mouse monoclonal antibody. Recognizes the ~50 kDa IL13RA2 protein.
Recognizes the ~50 kDa IL13RA2 protein in HeLa cells.
This Anti-IL13RA2 Mouse mAb (2E10) is validated for use in ELISA, Immunoblotting for the detection of IL13RA2.
Immunogen
A recombinant polypeptide corresponding to amino acids 27-127 of human IL13RA2, expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Zeis, T., et al. 2008. Brain131 288.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)