Merck, AP1167-100UG, Anti-TNS4 Mouse mAb (3B8)

General description
Purified mouse monoclonal antibody. Recognizes the ~52 kDa TNS4 protein.
Recognizes the ~52 kDa TNS4 protein in HeLa cells.
This Anti-TNS4 Mouse mAb (3B8) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of TNS4.
Immunogen
Full-length, recombinant human TNS4 (aa 1-481), expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Sakashita, K., et al. 2008. Ann Surg Oncol.15, 2606.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)