Merck, AP147B, Rabbit Anti-Sheep IgG Antibody, biotin-SP conjugate

Immunogen
Purified Sheep IgG.
Application
DILUTION:

1:50,000 - 1:1,000,000 for ELISA using enzyme-conjugated streptavidin

1:10,000 - 1:100,000 for Western Blots using enzyme-conjugated streptavidin

1:500 - 1:5,000 for enzyme immunohistochemistry on tissue sections

1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry

Optimal working dilution must be determined by the end user.
Rabbit anti-Sheep IgG Antibody, biotin-SP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Biochem/physiol Actions
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum protein, but the antibody may cross-react with immunoglobulins from other species.
Physical form
ImmunoAffinity Purified
Lyophilized. Buffer = 0.01M Phosphate buffer, 0.25 M NaCl with 15 mg/mL BSA and 0.05% sodium azide.
Preparation Note
Maintain lyophilized product at 2-8°C for up to 12 months from date of receipt. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Expiration date: one year from date of reconstitution.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.