Merck, AP181B, Goat Anti-Mouse IgG Antibody, biotin-SP conjugate, Species Adsorbed

Application
Detect Mouse IgG using this Goat anti-Mouse IgG Antibody, biotin-SP conjugate, Species Adsorbed validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications:

1:50,000-1:1000,000 for ELISA

1:10,000-1:1000,000 for Western blots

1:500-1:5000 for immunohistochemistry

1:200-1:1000 for FACS
Biochem/physiol Actions
Mouse IgG (H+L) (absorbed for minimal cross-reactivity to Rat, Human, Bovine and Horse Serum Proteins)
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6, with 0.05% Sodium Azide.

RECONSTITUTION:

Reconstitute with sterile distilled water.
Preparation Note
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.