Merck, AP502B, Goat Anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed

General description
Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Immunogen
Epitope: Ig kappa chain
Prepared from pooled antisera from goats hyperimmunized with human myeloma proteins with κ light chains.
Application
Immunofluorescence: ≤ 1 μg/10⁶ cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Fragment Specific Secondary Antibodies
This Goat anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed is validated for use in ELISA, IF for the detection of Human Ig κ chain.
Biochem/physiol Actions
Reacts with human kappa light chains. Absorbed for human myeloma proteins with λ light chains and mouse immunoglobulins.
The antibody reacts with human kappa light chains as demonstrated by ELISA and flow cytometry.
Physical form
Affinity Chromatography
Purified by affinity chromatography on pooled human Igs with κ light chains covalently linked to agarose. Liquid in 2.0 mL PBS/NaN₃.
Preparation Note
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.