Merck, AQ503B, Goat Anti-Human IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate

General description
IgG is the most abundant immunoglobulin comprising two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
Immunogen
Epitope: Human IgM, IgG, IgA
Prepared from pepsin digest of goat anti-human Ig.
Application
Goat anti-Human IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate is an antibody against Human IgM+IgG+IgA for use in ELISA & IF.
Immunofluorescence: ≤1 μg/10⁶ cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Biochem/physiol Actions
Reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD.
The antibody reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD as demonstrated by ELISA and flow cytometry.
Physical form
Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN₃.
Preparation Note
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.