Merck, B0939-10UG, BAFF active human

General description
BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.
Physical form
Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl.
Preparation Note
Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.
Analysis Note
The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED₅₀ ≤ 50 ng/mL
Other Notes
Extinction Coefficient: E⁰.¹% = 0.791 (A₂₈₀ nm)
p.I: 6.03