Merck, B2551-500G, Brewer thioglycollate medium

Application
Used for testing the sterility of biological products.
Preparation Note
Suspend 40.5 g of Brewer Thioglycollate Medium in 1000 ml of distilled water. Boil to dissolve the medium completely. Dispense in tubes or in suitable containers as desired and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.Note: If more than the upper one third of medium has acquired a green color, the medium may be restored once by heating on a water bath or free flowing steam until the green color disappears.
Other Notes
Components (g/L)
Proteose Peptone 10.00
Infusion from Beef 17.50
Dextrose 5.00
Sodium Chloride 5.00
Dipotassium Phosphate 2.00
Sodium Thioglycollate 0.50
Methylene Blue 0.002
Agar 0.50
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany