Merck, B9754, BIS-TRIS

General description
Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.
Application
Bis-Tris has been used :
as a buffer for Electrophoresis
as a component of transfer buffer for Western Blotting

Features and Benefits
Suitable for Biological and Biochemical Research
Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

Other Notes
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