Description
General description
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Application
Primary Human Coronary Artery Smooth Muscle Cells (HCASMC) are isolated from human coronary arteries and are provided in a proliferating format. HCASMC stain positive for smooth muscle α-actin. Coronary arteries supply the heart with blood and obstruction in atherosclerosis causes myocardial infarction.HCASMC are qualified to study the function of smooth muscle cells in the development of atherosclerotic plaques or in other diseases like hypertension. They are also useful for stent-graft compatibility testing.
Biochem/physiol Actions
Artery
Preparation Note
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Analysis Note
Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15
Recommended Primary Cell Culture Media:Link
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
