Description
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Application
Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request) and are provided in a proliferating format. PromoCell HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).
Biochem/physiol Actions
Cartilage
Preparation Note
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Analysis Note
Rigid quality control tests are performed for each lot of Human Chondrocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, each lot is routinely tested for the capacity to differentiate in 3D spheroids. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV and HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: Differentiation testedGuaranteed population doublings: >10
Recommended Primary Cell Culture Media:Link
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
