General descriptionEndothelial Cell Growth Medium 2 SupplementMix (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-22211
ApplicationEndothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
PackagingPart of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Preparation NoteClick
here for more information.
Final supplement concentrations after addition to the basal medium:Epidermal Growth Factor (recombinant human): 5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 10 ng / ml Insulin-like Growth Factor (Long R3 IGF): 20 ng / ml Vascular Endothelial Growth Factor 165 (recombinant human): 0.5 ng / mlAscorbic Acid: 1 µg / mlHeparin: 22.5 µg / ml Hydrocortisone: 0.2 µg / ml
Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Analysis NoteAll lots of PromoCell Endothelial Cell Media 2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
