Merck, C8274-250MG, Cytohelicase from Helix pomatia

Application
Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.
Cytohelicase from Helix pomatia has been used:
in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)
in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers
in the enzyme mix for the analysis of mitosis in anthers

Biochem/physiol Actions
Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall. Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.
Analysis Note
Contains glucanase/laminarinase activity