Merck, C9249-100ML, CryoSOfree™ DMSO-free Cryopreservation Medium

General description
Cultured cells in a suspension can be cryopreserved with minimal damage from freezing and thawing steps. CryoSOfree™ DMSO-free cryopreservation medium preserves them with high viability and physiological activity. This medium is recommended for the preservation of stem cells, hepatocytes, tissue sample and a wide variety of cell types and established cell lines. CryoSOfree™ DMSO-free cryopreservation medium is a proprietary formulation, containing no DMSO nor animal proteins. It is ready-to-use and complete with no additives required.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of DMSO for cryopreservation of cell cultures. For more information see here.
Application
CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown to preserve the potential of stem cell for differentiation.
Features and Benefits
Preserves pluripotency of stem cells
Low cytotoxicity
Chemically defined, serum free, protein free
Ready to use

The following cell lines have been recovered at ≥90% following cryopreservation with CryoSOfree:
L929, KB, MC3T3, B16F1, Colon26, CaCO2, HT1080, MG63
Preparation Note
1 ml of CyroSOfree is sufficient for 5x105- 5x106 cells.
Legal Information
CryoSOfree is a trademark of Sigma-Aldrich Co. LLC